human uterine leiomyosarcoma cell line  (ATCC)

Journal: Experimental and Therapeutic Medicine

Article Title: New treatment strategies for uterine sarcoma using secreted frizzled‑related proteins

doi: 10.3892/etm.2024.12520

Figure Lengend Snippet: SFRP4 was used to treat uterine leiomyosarcoma SKN cells at concentrations of 1.25 and 2.5 µg/ml, and the absorbance was measured. Cell viability was significantly suppressed in response to SFRP4 at a concentration of 2.5 µg/ml (n=8). *P=0.001 vs. the control. SFRP, secreted frizzled-related protein.

Article Snippet: Cell line and culture The SKN human uterine leiomyosarcoma cell line (European Collection of Authenticated Cell Cultures) was cultured in Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific, Inc.), penicillin (100 U/ml) and streptomycin (100 μg/ml) in an incubator containing 5% CO 2 at 37 ̊C.

Techniques: Concentration Assay, Control

Journal: Experimental and Therapeutic Medicine

Article Title: New treatment strategies for uterine sarcoma using secreted frizzled‑related proteins

doi: 10.3892/etm.2024.12520

Figure Lengend Snippet: Uterine leiomyosarcoma SKN cells were cultured in the CytoSelect 24-well Cell Migration Assay plates, SFRP4 was added at a concentration of 2.5 µg/ml, and the absorbance was measured. The ‘0’ group was the SFRP4 untreated control group. (A) Leiomyosarcoma cells showed a significant decrease in migration (n=4). (B) A histological image comparing SFRP4-untreated cells and SFRP-treated (2.5 µg/ml) cells. A decrease in cell number was observed in the treated group compared with the untreated group. Magnification, x40. *P=0.007 vs. control. SFRP, secreted frizzled-related protein.

Article Snippet: Cell line and culture The SKN human uterine leiomyosarcoma cell line (European Collection of Authenticated Cell Cultures) was cultured in Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific, Inc.), penicillin (100 U/ml) and streptomycin (100 μg/ml) in an incubator containing 5% CO 2 at 37 ̊C.

Techniques: Cell Culture, Cell Migration Assay, Concentration Assay, Control, Migration

Journal: Experimental and Therapeutic Medicine

Article Title: New treatment strategies for uterine sarcoma using secreted frizzled‑related proteins

doi: 10.3892/etm.2024.12520

Figure Lengend Snippet: Uterine leiomyosarcoma SKN cells were cultured in CytoSelect 48-well Cell Adhesion Assay plates, SFRP4 was added at a concentration of 2.5 µg/ml, and the absorbance was measured. The ‘0’ group was the SFRP4 untreated control group. (A) Adhesion ability of the leiomyosarcoma cells was significantly increased to each cell adhesion molecule (n=4). (B) A histological image comparing SFRP4-untreated cells and SFRP-treated (2.5 µg/ml) cells. An increase in cell number was observed in the treated group compared with the untreated group. Magnification, x40. *P<0.01 vs. control. Col, collagen; Lam, laminin I; SFRP, secreted frizzled-related protein.

Article Snippet: Cell line and culture The SKN human uterine leiomyosarcoma cell line (European Collection of Authenticated Cell Cultures) was cultured in Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher Scientific, Inc.), penicillin (100 U/ml) and streptomycin (100 μg/ml) in an incubator containing 5% CO 2 at 37 ̊C.

Techniques: Cell Culture, Cell Adhesion Assay, Concentration Assay, Control